HEMODILUTION PRINCIPLE OF HYPOTHERMIC PERFUSION

The technique of hypothermic perfusion.

The machine described is in commercial production although the perfusions, to date, were performed with the prototype described by Ross (1960a). The extracorporeal circuit is mounted on the front of the machine (Fig. 1) under the vision of the perfusionist and comprises: Coronary suction reservoir (A) into which all wound and coronary sinus blood is returned by suction from a horizontally mount...

Clinical studies of hypothermic perfusion by the hemodilution technique, especially its influence on water and electrolytes changes, and renal function.

Hypothermic low flow cardiopulmonary bypass with hemodilution technique was performed in open-heart surgery in the author's clinic. To evaluate the usefulness of this perfusicn, renal hemodynamics during total bypass, and its influence on renal function were studied. The average effective renal blood flow during total bypass at the perfusion rate of 50 to 60 ml per kg per minute under mild or m...

Hypothermic Machine Perfusion of Composite Tissues

Effect of hypothermic perfusion on corneal endothelial morphology.

The effect of moderate in-vivo hypothermic perfusion on corneal endothelial integrity was studied in the cat. Eleven cats underwent in-vivo anterior chamber perfusion for 30 minutes with either normothermic (23 degrees C) or hypothermic (5 degrees C) perfusate. Corneas were then evaluated clinically (biomicroscopy), functionally (vital staining), and morphologically (scanning electron microscop...

Hypothermic Machine Perfusion Preservation of the DCD Kidney: Machine Effects

Purpose. Kidneys from DCD donors represent a significant pool, but preservation problems exist. The study objective was to test the importance of machine type for hypothermic preservation of DCD kidneys. Methods. Adult Beagle dog kidneys underwent 45 minutes of warm in situ ischemia followed by hypothermic perfusion for 24 hours (Belzer-MPS Solution) on either an ORS LifePort or a Waters RM3 us...